受体阻断剂改善家兔急性脑缺血的研究

利用家兔制备急件不完全世脑缺血模型，选择Ｒｅｇｉｔｉｎｅ作为α－子体阻断剂，通过静脉给药，旨在探寻临床急性脑缺血状态下改善脑血流量的救治方法。结果表明，静注Ｒｅｇｉｔｉｎｅ后，可使脑血管迅速舒张，脑血流量有效增加，平均动脉压可稳定在一定水平，心电图ＳＴ段和心率无显著改变。     

拮抗细菌Enterobacter cloaeae B8的一株抗菌素抗性突变体(英文)

为了研究拮抗细菌Enterobacter cloacae B8在野外水稻叶面上的定殖情况及拮抗作用,由自然选择和Tn5诱变从B8产生了一株抗菌素抗性突变体BX8.BX8能在含利福平达400μg/ml或卡那霉素达300μg/ml的LB培养基中生长.实验表明该抗菌素抗性的产生没有降低BX8的拮抗活性.该抗性比较稳定,在无抗菌素培养基中培养.BX8至少分裂生长60代内抗性不变.     

原油等熵压缩温升测定及研究

采用反平衡法测试泵的效率，具有测试参数少（不需要测定泵流量），易于实现等优点，但是原油等熵压缩温升参数及其测试方法需要深入研究。实测液体等熵压缩温升要在绝热，压力稳定的条件下进行，并取升压测得的温升△ｔｐ和降压测得的温降△ｔｐ的算术平均值，作为等熵温升。从对原油实测的等熵压缩温升结果来看，原油的等熵压缩温升与原油性质，被压缩原油的温度，压力，密度有关，其中与原油密度的关系较为明显。介绍了测试这一参     

脊髓CGRP在大鼠实验性关节炎形成和发展中的作用

采用鞘内注射降钙素基因相关肽（ＣＧＲＰ）受体拮抗剂人ＣＧＲＰ片段８－３７（ｈＣＧＲＰ８－３７），探讨了佐剂性关节炎大鼠初级感觉系统中ＣＧＲＰ增多的意义。结果：佐剂前注射ｈＣＧＲＰ８－３７的动物，在注射佐剂后１～２０ｄ，患肢及健肢皮温均高于对照组（Ｐ＜０．０５）；患肢足容积明显大于对照组（Ｐ＜０．０５），并且在形成变态反应性佐剂性关节炎时（佐剂后１３～２０ｄ），健肢足容积也明显增加（Ｐ＜０．０５）；但双后肢的痛阈变化与对照组相比没有明显差异（Ｐ＞０．０５）。上述结果表明，佐剂前鞘内注射ｈＣＧＲＰ８－３７，使急性关节炎和多发性关节炎均有所加重。佐剂后１２ｄ注射ｈＣ－ＧＲＰ８－３７的动物，双后肢的肿胀积分均明显高于对照组（佐剂后１８～５０ｄ，Ｐ＜０．０５或０．０１），痛级别也明显高于对照组（佐剂后１４～５０ｄ，Ｐ＜０．０１），但痛阈的变化不明显（Ｐ＞０．０５）。因此佐剂后１２ｄ鞘内注射ＣＧＲＰ的拮抗剂也可加重关节炎的炎症反应。这进一步证明了脊髓ＣＧＲＰ可能具有抗炎和抑制免疫反应的作用。     

PULSED WAVE-DOPPLER ULTRASONOGRAPHIC EVALUATION OF THE COMMON CAROTID ARTERY IN THE RESTING HORSE: PHYSIOLOGIC DATA

A pulsed wave-Doppler ultrasonographic evaluation of common carotid arterial blood flow was carried out on 63 healthy Italian Saddlebred horses. Vessel diameter and tracing morphology were evaluated were evaluated and blood flow parameters (systolic, diastolic and mean velocity, acceleration and deceleration of the systolic wave, cartid pulse volume) were calculated and correlated with class variables (sex, age and body weight). On the basis of the presence of an incisure in the ascending branch of the systolic curve, subjects were divided in two groups: one with a bifid systolic curve and the other with a monophasic aspect. Correlations between: 1) diameter of the vessel and body weight and 2) carotid pulse volume and flow velocity (systolic, diastolic and mean) were found. A greater systolic pulse volume was found in male subjects, in subjects with greater body weight and in those which had a monophasic systolic wave.     

田山一级泵站技术改造浅析

田山电灌工程是黄河下游山东境内最大的电力引黄提灌工程。文章介绍了田山泵站前池跃前底部消能、跃后上方消波和轴流泵清水润滑新装置,以及提高泵站电力功率因数综合性水泵汽蚀防护和技术改造措施,经济效益显著。对泵站建设和技术改造具有一定的实用价值。     

绘制天然河道水面曲线的图解计算法

本文根据流量模数的倒数曲线法,利用合轴图及数值叠加规则,提出了一种适用于山区河道的图解计算方法,本法解算过程较为直观,便于检查正误,计算精度较高,特别是在需要绘制一系列流量水面曲线的情况下,可加快计算的进度。     

The in vitro cytotoxicity of native and modified trichloroacetic acid-extracted antigens from Fusobacterium necrophorum for mouse peritoneal macrophages

A Boivin lipopolysaccharide-protein complex (LPS-P), Westphal LPS (W-LPS), Boivin LPS (B-LPS), and a lipid A-associated protein (LAP) were extracted from Fusobacterium necrophorum bov 5 strain and chemically analyzed for protein, total nitrogen, hydrolyzed hexosamine, and nucleic acid content. Dose and time effects on the in vitro cytotoxicity of these preparations for mouse peritoneal macrophages were determined. Macrophage monolayers were exposed to 2.0 ml of 10, 100, 250, 500, or 1000 μg/ml solutions of the individual preparations for 1, 2, 4, or 6 hr at 37°C, and relative toxicities were determined; B-LPS> LPS-P> LAP, W-LPS. The effects of heat, NaOH, and trypsin and pronase on the native antigens were determined. Heat treatment had no effect on the cytotoxicity of B-LPS, but increased the toxicity of LPS-P and LAP for macrophages. Alkaline treatment decreased the cytotoxicity of B-LPS and increased the toxicity of LPS-P and LAP for macrophages. Enzyme treatment had no effect on LPS-P toxicity. The relationship of lipid A and protein content to toxicity for macrophages is discussed. Lipid A appears to be important in the cytotoxicity of B-LPS, and the protein components of LPS-P and LAP may contribute to their toxic activity. The ability of these bacterial cellular antigens to destroy phagocytic cells may facilitate the establishment of necrotic abscesses in susceptible hosts.     

Demonstration of inflammatory cell population changes in rat lungs in response to intratracheal instillation of spring wheat dust using lung enzymatic digestion and centrifugal elutriation

The inhalation of grain dust by grain workers is responsible for a large number of pulmonary pathophysiologies. These problems may be acute or chronic and may be mediated by the chronic activation of the immune system. Constant inflammatory states in the lung may eventually lead to tissue damage and respiratory deficit. This study was designed to measure the changes in the relative number of inflammatory cells in peripheral blood, bronchoalveolar spaces, and lung interstitium that occur in response to intratracheally instilled airborne spring wheat dust in rats. It was found that 6h after instillation with dust, neutrophils were present in greater numbers in the blood and bronchoalveolar spaces than in lung interstitium. After 24h, there appeared to be a larger number of neutrophils in the lung interstitium in dust-instilled animals than in saline-instilled controls. These results indicate that intratracheal instillation of grain dust initiates an acute inflammatory reaction, and that there is an initial influx of neutrophils into the air spaces of the lung followed by transit of these cells into the lung interstitium.     

Comparison of various tests for the serological diagnosis of Trypanosoma equiperdum infection in the horse

Comparative tests such as FAT, ELISA, RIA, IEO and CF in the diagnosis of dourine in the horse have proved a satisfactory concordance ratio of the ELISA with CF, which seems to be the most reliable test. Discrepancies have been observed as to the other tests which appear less sensitive than CF test.